Use standard laboratory procedures, like cell staining, culturing and DNA sequencing to further narrow down your identification. Bacteria are generally grouped into species according to their physical and metabolic characteristics, rather than their genetic relationships to one another.
Experiment for identification of unknown bacteria! Identification of unknown bacteria is one of the major responsibilities of the microbiologists. Samples of blood, tissue, food, water and cosmetics are examined daily in laboratories throughout the world for the presence of contaminating microorganisms.
In addition, industrial Unknown bacteria identification are constantly screening materials to isolate new antibiotic-producing microbes or microbes that will increase the yield of marketable products, such as vitamins, solvents and enzymes.
Once isolated, these unknown microbes must be identified and classified. The science of classification is called taxonomy and deals with the separation of living organisms into interrelated groups.
With the fundamental knowledge in staining methods, isolation techniques, bacterial nutrition, biochemical activities and growth characteristics of bacteria, it becomes easier for identification of any unknown bacteria. Characteristics of few bacteria have been given in Table 7.
Other bacteria can be identified in a similar way based on the observations and results obtained following the experimental procedures. Slides, cavity slides, petri dishes, conical flasks, cotton plugs, inoculating loop, autoclave, bunsen burner, laminar flow chamber, dispose jar, incubator, nutrient broth, nutrient agar, gram staining reagents, media and reagents for biochemical tests, compound microscope, isolated colonies or pure cultures of bacteria.
Gram staining of the unknown bacteria is performed. Besides gram staining, its morphology and arrangement is also recorded. Motility test of the bacteria is determined by hanging drop preparation Figure 7.
Using sterile inoculating technique, the bacteria is inoculated into two nutrient agar slants, a nutrient broth tube and to a nutrient agar plate by means of streak inoculation. Following incubation, one slant culture is used to determine the cultural characteristics of the unknown bacteria.
The second is used as a stock sub-culture, should it be necessary to repeat any of the tests. Growth characteristics are also observed in the broth tube and colony characteristics on the plate.
Exercising care in sterile technique, so as not to contaminate cultures and thereby obtain spurious results, the bacteria is inoculated into respective media to carry out the different biochemical tests. The inoculated media are incubated at the required temperatures for the required lengths of time.
In gram staining, besides gram reaction, the morphology and arrangement of the bacteria are also recorded. The cultural characteristics of the bacteria in nutrient broth, on agar slant and on nutrient agar plate are noted. Results of the biochemical reactions are recorded. Using the above recorded data For example, see Table 7.Identification of unknown bacteria is one of the major responsibilities of the microbiologists.
Samples of blood, tissue, food, water and cosmetics are examined daily in laboratories throughout the world for the presence of contaminating microorganisms. In addition, industrial organisations are. Bacteria may seem like very simple organisms and you might think that most of them share many characteristics.
In fact, every species is unique and has particular characteristics. This makes it possible to identify an unknown species. IDENTAX bacterial identifier is a free program for the taxonomical identification of bacteria using phenotypical characteristics.
It is aimed at the microbiologist community, and also at any field with a need for cheap and fast classification of unknown strains of bacteria.
Identifying the unknown bacterium was determined by separating and differentiating possible bacteria based on specific biochemical characteristics.
The differential tests used to identify the unknown cultures were Gram staining, oxidase, indole test, urea test, and casein test.
Before going over the tests to identify an unknown bacterial species, we should remember some bases of manipulating bacteria. It is important to always keep in mind that your unknown species is a potential pathogen. To identify the bacterial unknowns in a mixed culture by morphological and biochemical methods. Principle: The identification of bacteria is a careful and systematic process that uses many different techniques to narrow down the types of bacteria that are present in an unknown bacterial culture. Begin the process of identifying unknown bacteria by observing their physical characteristics, such as cell wall, shape and linkages. Use standard laboratory procedures, like cell staining, culturing and DNA sequencing to further narrow down your identification.
Begin the process of identifying unknown bacteria by observing their physical characteristics, such as cell wall, shape and linkages.
Use standard laboratory procedures, like cell staining, culturing and DNA sequencing to further narrow down your identification.
Identification of unknown bacteria by the manual method using staining reactions, motility test, cultural characteristics and biochemical tests is a cumbersome, lengthy and time-consuming process. To make the process easy and short, automatic methods have been developed.